Transgenics & Other Animal Services
Transgenics & Other Animal Services
Penn State College of Medicine offers accredited services in the generation of transgenic and knockout mice and in rederivation. The College is also home to a zebrafish facility (opened in 2016) and a pulmonary immunology and physiology facility (opened in 2015).
- Zebrafish Functional Genomics Core
- Pulmonary Immunology & Physiology Core
- Transgenics & Rederivation
- Animal Pathology
Zebrafish Functional Genomics Core
The Zebrafish Functional Genomics Core at Penn State College of Medicine was established to provide the Penn State research community with a modern, centralized facility for housing, breeding and performing experiments with zebrafish, one of the fastest growing model systems in biomedical research, as well as the intellectual infrastructure to do that work.
About the Facility
The central housing room is equipped with 32 racks of recirculating aquaria, able to accommodate any combination of 1, 3 and 10 liter tanks.
Other facilities include twin 16-rack recirculating systems with independent filtration/purification, an isolated quarantine room with three stand-alone housing systems, a sentinel pathogen-monitoring program, a procedure room and three independently controlled light-tight breeding cabinets.
The Zebrafish Functional Genomics Core provides a variety of instrumentation for microinjection and fluorescence imaging.
- Eppendorf FemtoJet microinjectors
- Manual micromanipulators
- ZEISS Stereo Discovery.V8 microscopes
- Leica MZ FLIII Fluorescence Stereomicroscope
- Olympus MVX10 MacroView Macro Zoom Fluorescence Microscope
- ZEISS Axio Zoom.V16 Zoom Fluorescence Microscope with ZEISS ApoTome.2
Fish are available to both Penn State College of Medicine and external investigators. We currently house the following wild type lines: AB, Conner, Ekkwill, Liles, Tübingen (Tu) and WIK. We also house a selection of mutant and transgenic lines.
Pulmonary Immunology and Physiology Core
The Penn State College of Medicine Pulmonary Immunology and Physiology Core aids investigators in the development and testing of small-animal models to be used for pre-clinical pulmonary research.
The Pulmonary Immunology and Physiology Core is a BSL-2 facility that allows the assessment of pulmonary function related to many clinical situations including airway hyper-responsiveness, ventilation-induced lung injury, postnatal lung development, airway remodeling, chronic inflammation, allergy, environmental exposures and infection.
In addition, services offered include oxygen profiling, infection and treatment; the ability to deliver, measure and test the pre-clinical potential of therapeutic interventions via aerosol; and the housing of small animals used in these studies.
- SCIREQ flexiVent FX
- SCIREQ inExpose
- Data Sciences International FinePointe Whole Body Plethysmograph
- STARR Life Sciences MouseOx Plus
- BioSpherix OxyCycler A84X05
- Nuaire Biological Safety Cabinet
- flexiWare Software
- Data Sciences International FinePointe Software
Transgenics, Rederivation and Bioluminescence Imaging
The Transgenic Core at Penn State College of Medicine is a centralized facility for the production of transgenic and knockout mice and the rederivation of mice.
(Penn State Access ID login required)
Penn State College of Medicine's Department of Comparative Medicine offers a Comparative Pathology Service to provide researchers access to experts in veterinary and comparative pathology.
Services available include gross necropsy, histopathology (including semi-quantitative or quantitative analysis), immunohistochemistry, phenotyping, development/implementation of analysis techniques, manuscript review, and clinical pathology.
For details on the comparative pathology service, email Nannette Kirst in the Department of Comparative Medicine at firstname.lastname@example.org.