Flow Cytometry
FACSDiscover S8
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Overview
The FACSDiscover S8 instrument was purchased through an NIH S10 grant (S10OD038168).
This instrument combines spectral flow cyometry with real-time image-enabled sorting to identify and isolate unique cell populations based on high-parameter phenotypic and morphological characteristics, as well as cell subsets with desired marker localization.
Additional Details
Using 5 lasers, UV (349 nm), violet (405 nm), blue (488 nm), yellow-green (561 nm) and red (637 nm), in combination with an array of 78 fluorescent detectors the FACSDiscover provides maximum flexibility in fluorochrome choice and high-parameter marker detection.
Three fluorescent imaging detectors are available following excitation from the blue laser (FL1: 534/46; FL2: 600/60; FL3: 788/225) in addition to detection of forward scatter, side scatter and axial light loss. Multiple imaging features are available for real-time sorting and data analysis including Correlation, Delta center of mass, Diffusivity, Eccentricity, and Max intensity.
For cell sorting, 85 uM, 100 uM and 130 uM nozzles are available with up to 6-way sorting into 5 ml tubes or 2-way sorting into 15-ml or 50-ml tubes. Plate sorting and index sorting are available for 6-, 24-, 48-, 96- and 384-well plates.
Consultation
Contact Jade Vogel, Cell Sorting Specialist, for assistance with experimental design and sample preparation and biosafety procedures. (jvogel@pennstatehealth.psu.edu or x6908)
Contact Dr. Todd Schell for questions related to instrument capabilities and the use of the instrument to enhance your research approach. (tschell@pennstatehealth.psu.edu)
Schedule the FACSDiscover by accessing the reservation calendar.
Acknowledgement
Please use the following statement in the acknowledgements section of all publications that utilize the FACSDiscover S8 Instrument.
“Research reported in this publication was supported by the Office of The Director, National Institutes of Health under Award Number S10OD038168. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.”
Advisory Committee
Members of the S10 advisory committee provide oversight for the FACSDiscover S8 cell sorter. If you have concerns regarding access to the instrument and the services provided or have suggestions for improved operational procedures, please contact a member(s) of the committee to share your comments.
Shyam Bansal, PhD, Professor of Medicine (sbansal1@pennstatehealth.psu.edu)
Joseph Bednarczyk, non-voting member, Flow Cytometry Core Manager (jbednarczyk@pennstatehealth.psu.edu)
Maria Bewley, PhD, Senior Scientific Director, Core Facilities, Chair of committee (mbewley@pennstatehealth.psu.edu)
Edward Gunther, MD, Professor of Medicine (egunther@pennstatehealth.psu.edu)
Leslie Parent, MD, Professor of Medicine (lparent@pennstatehealth.psu.edu)
Todd Schell, PhD, non-voting member, Professor and Scientific Director, Flow Cytometry Core (tschell@pennstatehealth.psu.edu)
Hong-Gang Wang, PhD, Professor of Pediatrics (hwang3@pennstatehealth.psu.edu)