Pathology
Molecular and Histopathology Core
In This Section
The Molecular and Histopathology Core, part of the Department of Pathology and Laboratory Medicine, provides a broad array of state-of-the-art technical and professional pathology-related services critical to biomedical investigations conducted at Penn State College of Medicine and Penn State Health Milton S. Hershey Medical Center.
Services
Fixation and processing of human and animal tissues, as well as cultured cells and tissues Paraffin embedding and sectioning of tissues
Embedding and cryostat sectioning of fresh, frozen tissues
Routine H&E staining of human and animal tissues, as well as cultured cells and tissues
H&E staining for paraffin or frozen sections
Histochemical staining of paraffin or frozen tissue sections
PAS
Masson Trichrome
Toluidine Blue
Gomori’s Aldehyde Fuchsin
Safranin O
Verhoeff Elastic
Cresyl Violet
Ability to develop and optimize histochemical stains for any tissue type or staining need
Immunohistochemical staining oh Human and animal tissues, as well as cultured cells and tissues
Ability to validate and develop protocols for any antibody
Mouse-on-mouse/rat immunohistochemistry
Chromogenic and fluorescent detection methods
Dual and triple IHC techniques
All IHC performed on an automated Ventana Discovery XT stainer
Chromogenic or fluorescent in situ hybridization protocols
Ability to develop and optimize protocols for your specific probes
All hybridizations protocols performed on an automated Ventana Discovery XT stainer
Paraffin tissue microarrays (TMAs) can be created with any combination of human tissue, animal tissues
or cell culture pelletsCustom-sized and configured arrays are constructed using the Beecher MTA-I
Tissue core sizes can range from 0.6 mm to 2.0 mm, providing from 40 to over 200 sample cores in one
paraffin blockMicroarray slides can be stained using a variety of histochemical or immunohistochemical reactions
Zeiss PALM Microbeam LCM
Capable of isolating cells, tissues, or stromal matrix from either frozen and paraffin sections
Ideal method to insure the purity of the starting material for downstream proteomic and genomic applications
Capable of operating on an RNAse free level to insure high quality samples for genomic studies
Archival collection consisting of over 40 years of surgical pathology FFPE specimens
Searchable database enables the investigator to locate specific patient, tissue or tumor types
Ability to collaborate with anatomic pathologists to assist in the identification and selection of study samples along with the interpretation and scoring of results
Perfect for use as control tissues, study samples or TMA sources
Capable of isolating DNA or RNA from samples
