Aperio Slide Scanning Core

The Aperio Slide Scanning Core, part of the Department of Pathology and Laboratory Medicine, provides researchers the ability to image entire glass histology slides at either 20X or 40X while storing the data as a virtual slide. The core is equipped with an Aperio AT2 digital slide scanner, capable of delivering high-throughput whole slide imaging for up to 400 slides at a time.

The AT2 generates bright-field whole slide images at 20X (0.50µm/pixel) or 40X (0.25µm/pixel) magnification output as a 24-bit color pyramid TIFF (.SVS file). Once scanned, slides can be viewed as a virtual slide at any magnification up to the scanning resolution. Readily available Aperio software can be downloaded and used to view these virtual slides on your computer.

Features such as viewing, exporting, annotating, scale bars and image adjustments are available with the Aperio software. Researchers can then take their virtual slides back to their lab and apply the images to their lab’s image analysis pipeline, use them in publications, use them for teaching or simply place them in long term storage.

Instrumentation

Aperio AT2 Digital Slide Scanner: Bright-field digital slide scanning at 20X or 40X resolution to produce virtual slides.

Procedures, Protocols and Forms

Citation: Investigators should cite Penn State College of Medicine’s Aperio Slide scanning Core either in the Materials and Methods section or the Acknowledgements section of any article.

Working With the Aperio Scanning Core

Fees: For information on fees, contact the core.

Location: Aperio Slide Scanning Core is in Room C7716A at Penn State College of Medicine.

Publications

Computational 3D Histological Phenotyping of Whole Zebrafish by X-ray Histotomography. Ding Y, Vanselow DJ, Yakovlev MA, Katz SR, Lin AY, Clark DP, Vargas P, Xin X, Copper JE, Canfield VA, Ang KC, Wang Y, Xiao X, De Carlo F, van Rossum DB, La Riviere P, Cheng KC. Elife. 2019 May 7;8. pii: e44898.

Comparative analysis of fixation and embedding techniques for optimized histological preparation of zebrafish. Copper JE, Budgeon LR, Foutz CA, van Rossum DB, Vanselow DJ, Hubley MJ, Clark DP, Mandrell DT, Cheng KC. Comp Biochem Physiol C Toxicol Pharmacol. 2017 Nov 20. pii: S1532-0456(17)30208-9. doi:10.1016/j.cbpc.2017.11.003. PubMed PMID: 29157956, PMCID: PMC5936644.0.7554/eLife.44898 PMID: 31063133.

Contact the Core

Jean Copper, BA, MS, Core Manager

Email: jec13@psu.edu
Phone: 717-531-5301