Mass Spectrometry & Proteomics

Mass Spectrometry & Proteomics

Penn State College of Medicine's Mass Spectrometry & Proteomics Core provides mass spectrometry analyses and identification of proteins, peptides, oligonucleotides, carbohydrates and small molecules.

Other services include separations of complex protein and/or peptide mixtures; protein expression analysis (iTraq, SILAC, SWATH/DIA label-free); quantitation of protein, cytokine, amino acid and other small-molecule levels; bioinformatics; spot-cutting and robotics; and gel imaging and analysis.

Instrumentation

The following major equipment is available in the Mass Spectrometry & Proteomics Core:

  • Mass spectrometers
    • ABSciex TripleTOF 5600+ mass spec
    • ABI/MDS Sciex 5800 MALDI TOF-TOF mass spec
    • MDS Sciex 4000 QTrap Hybrid Ion Trap mass spec
    • Waters Synapt HDMS Hybrid QTOF with ion mobility
    • ABI Voyager DE-PRO Reflectron MALDI TOF mass spec
  • Plate readers for quantitation of chemokines, peptide hormones, growth factors
    • MesoScale Discoveries (MSD) QuickPlex SQ 120 electrochemiluminescence detector/plate reader
    • MesoScale Discoveries (MSD) Sector Imager 2400 electrochemiluminescence detector/plate reader
  • HPLC/UPLC and gel separation systems
  • Gel imaging and band/spot quantitation analysis tools
  • Shimadzu CHiP printer
  • Wyatt DAWN HELEOS light-scattering detection system (for protein sizing)

Contact Us

For details on the Mass Spectrometry & Proteomics Core, email Bruce Stanley, Director, at bstanley@psu.edu or call 717-531-5329.