Core Facilities Genomic Analysis Genome Sciences Facility Tab Code
Genome Sciences Facility
The full-service Penn State College of Medicine Genome Sciences Facility provides consultation, instrumentation and services to both Penn State and non-Penn State investigators in genomic, epigenomic and transcriptomic studies.
The variety of instrumentation allows for capabilities ranging from highly focused analysis of candidate SNPs and mRNAs to whole genome, exome, epigenome and transcriptome sequencing. Services are also available for a variety of study designs extending from a few laboratory samples to large clinical projects involving hundreds or thousands of samples. The full bioinformatics service is also available for data analysis.
About the Genome Sciences Facility
The facility resides in 5,000 square feet of newly renovated space, encompassing separate "pre-amplification" and "post-amplification" rooms to prevent any contamination of PCR-amplified materials to pre-processed input DNA/RNA samples. Four well-experienced staff members are available for assisting in project operations. In addition, the lab space is available for investigators who need temporary room for sample preparation.
We receive either tissue, DNA/RNA, or customer-generated NGS libraries. We process samples accordingly based on agreement reached during consultations on the design of the experiment. We develop new applications to accommodate state-of-the-art NGS technologies. We conduct sequence read alignment, secondary analysis (quantitation, variant calling, functional annotation, visualization, etc) and follow-up with the interpretation of the results. We provide support for grant writing and hands-on training for students and post-docs in NGS processing.
A variety of instrumentation is available in the Genome Sciences Facility for sample processing, sample quantitation and quality control, qPCR/digital PCR, microarray analyses, next-generation sequencing and other needs.
- QIA Symphony DNA/RNA Extraction Robot
- Bullet Blender (high-throughput tissue homogenizer)
- Covaris Adaptive Focused Acoustics Ultrasonicator E-Series
Sample Quantitation and Quality Control
- Agilent 2100 Bioanalyzer
- Nanodrop ND-1000 Spectrophotometer
- Qubit Fluorometer
- Molecular Devices UV/Vis/Fluor Spectrophotometer (available in Drug Discovery Core)
- ABI QuantStudio 12K Flex system with available OpenArray block
- ABI QuantStudio 3D Digital PCR System
Next-Generation Sequencing (NGS)
- Illumina GenomeStudio
- Illumina iCompute
- Ingenuity Pathway Analysis
For Ingenuity Pathway Analysis access information, please contact the IS Service Desk at 1-833-577-HELP (4357) or firstname.lastname@example.org.
Additionally the facility contains refrigerators, -20ºC and -80ºC freezers, and associated small equipment (e.g. multichannel, electronic, and accordion pipettes, centrifuges, etc.).
For details on the Genome Sciences Facility, email email@example.com or call 717-531-5823.