Genome Sciences Facility

The variety of instrumentation allows for capabilities ranging from highly focused analysis of candidate SNPs and mRNAs to whole genome, exome, epigenome and transcriptome sequencing. Services are also available for a variety of study designs extending from a few laboratory samples to large clinical projects involving hundreds or thousands of samples. The full bioinformatics service is also available for data analysis.

About the Genome Sciences Facility

The facility resides in 5,000 square feet of newly renovated space, encompassing separate "pre-amplification" and "post-amplification" rooms to prevent any contamination of PCR-amplified materials to pre-processed input DNA/RNA samples. Four well-experienced staff members are available for assisting in project operations. In addition, the lab space is available for investigators who need temporary room for sample preparation.

We receive either tissue, DNA/RNA, or customer-generated NGS libraries. We process samples accordingly based on agreement reached during consultations on the design of the experiment. We develop new applications to accommodate state-of-the-art NGS technologies. We conduct sequence read alignment, secondary analysis (quantitation, variant calling, functional annotation, visualization, etc) and follow-up with the interpretation of the results. We provide support for grant writing and hands-on training for students and post-docs in NGS processing.

Instrumentation

A variety of instrumentation is available in the Genome Sciences Facility for sample processing, sample quantitation and quality control, qPCR/digital PCR, microarray analyses, next-generation sequencing and other needs.

Sample Processing

• QIA Symphony DNA/RNA Extraction Robot
• Bullet Blender (high-throughput tissue homogenizer)
• Covaris Adaptive Focused Acoustics Ultrasonicator E-Series

Sample Quantitation and Quality Control

• Agilent 2100 Bioanalyzer
• Nanodrop ND-1000 Spectrophotometer
• Qubit Fluorometer
• Molecular Devices UV/Vis/Fluor Spectrophotometer (available in Drug Discovery Core)

qPCR/Digital PCR

• ABI QuantStudio 12K Flex system with available OpenArray block
• ABI QuantStudio 3D Digital PCR System

Microarray Analyses

• Illumina iScan

Next-Generation Sequencing (NGS)

• Illumina MiSeq
• Illumina NovaSeq

Software

• Illumina GenomeStudio
• Illumina iCompute
• Ingenuity Pathway Analysis

For Ingenuity Pathway Analysis access information, please contact the IS Service Desk at 1-833-577-HELP (4357) or help@pennstatehealth.psu.edu.

Additionally the facility contains refrigerators, -20ºC and -80ºC freezers, and associated small equipment (e.g. multichannel, electronic, and accordion pipettes, centrifuges, etc.).

Contact Us

For details on the Genome Sciences Facility, email genomesciences@pennstatehealth.psu.edu or call 717-531-5823.

Zebrafish Functional Genomics Core

About the Facility

The Zebrafish Functional Genomics Core is a newly designed facility housing a state-of-the-art Pentair Aquatic Habitats recirculating aquaria, housed in the Department of Comparative Medicine.

The central housing room is equipped with 32 racks of recirculating aquaria, able to accommodate any combination of 1, 3 and 10 liter tanks.

Other facilities include twin 16-rack recirculating systems with independent filtration/purification, an isolated quarantine room with three stand-alone housing systems, a sentinel pathogen-monitoring program, a procedure room and three independently controlled light-tight breeding cabinets.

See photos of the facility taken during its April 2016 grand opening.

Instrumentation

The Zebrafish Functional Genomics Core provides a variety of instrumentation for microinjection and fluorescence imaging.

Microinjection

• Eppendorf FemtoJet microinjectors
• Manual micromanipulators
• ZEISS Stereo Discovery.V8 microscopes

Fluorescence Imaging

• Leica MZ FLIII Fluorescence Stereomicroscope
• Olympus MVX10 MacroView Macro Zoom Fluorescence Microscope
• ZEISS Axio Zoom.V16 Zoom Fluorescence Microscope with ZEISS ApoTome.2

Fish

Fish are available to both Penn State College of Medicine and external investigators. We currently house the following wild type lines: AB, Conner, Ekkwill, Liles, Tübingen (Tu) and WIK. We also house a selection of mutant and transgenic lines.

Contact Us

For details on the Zebrafish Functional Genomics Core, email Khai Chung Ang, PhD, Scientific Director, at kca2@psu.edu or call 717-531-4704.

Macromolecular Synthesis Core

About the Facility

The Macromolecular Synthesis Core Facility is heavily used, and generates approximately 2,000 oligonucleotides and 100 peptides per year to investigators around the US. Funding from NIH, NSF, Pennsylvania Department of Health Tobacco Settlement Funds (CURE) and State of Pennsylvania grants have helped to provide instrumentation for these services.

Services

• Peptide Synthesis
• Oligonucleotide Synthesis

Contact Us

For details on the Macromolecular Synthesis Core, email Anne Stanley, Lab Manager and Senior Research Support Associate, at astanley@psu.edu or call 717-531-6087.