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Transgenics & Other Animal Services

Transgenics & Other Animal Services

Penn State College of Medicine offers accredited services in the generation of transgenic and knockout mice and in rederivation. The College is also home to a zebrafish facility (opened in 2016) and a pulmonary immunology and physiology facility (opened in 2015).

The laboratory animal medicine programs at Penn State College of Medicine are accredited through a number of major organizations, including AAALAC International, AALAS, ACLAM, ASLAP, AAVMC and PSBR.

Zebrafish Functional Genomics Core

The Zebrafish Functional Genomics Core at Penn State College of Medicine was established to provide the Penn State research community with a modern, centralized facility for housing, breeding and performing experiments with zebrafish, one of the fastest growing model systems in biomedical research, as well as the intellectual infrastructure to do that work.

About the Facility

The Zebrafish Functional Genomics Core is a newly designed facility housing a state-of-the-art Pentair Aquatic Habitats recirculating aquaria, housed in the Department of Comparative Medicine.

The central housing room is equipped with 32 racks of recirculating aquaria, able to accommodate any combination of 1, 3 and 10 liter tanks.

Other facilities include twin 16-rack recirculating systems with independent filtration/purification, an isolated quarantine room with three stand-alone housing systems, a sentinel pathogen-monitoring program, a procedure room and three independently controlled light-tight breeding cabinets.

See photos of the facility taken during its April 2016 grand opening.

This image of the Zebrafish Functional Genomics Core at Penn State College of Medicine, taken during its April 2016 grand opening, shows an aisle framed by five shelves of zebrafish tanks down either side. The tanks appear deep blue, and zebrafish can faintly be seen swimming in the frontmost ones.
Penn State College of Medicine's Zebrafish Functional Genomics Core opened in April 2016.

Instrumentation

The Zebrafish Functional Genomics Core provides a variety of instrumentation for microinjection and fluorescence imaging.

Microinjection

  • Eppendorf FemtoJet microinjectors
  • Manual micromanipulators
  • ZEISS Stereo Discovery.V8 microscopes

Fluorescence Imaging

  • Leica MZ FLIII Fluorescence Stereomicroscope
  • Olympus MVX10 MacroView Macro Zoom Fluorescence Microscope
  • ZEISS Axio Zoom.V16 Zoom Fluorescence Microscope with ZEISS ApoTome.2

Fish

Fish are available to both Penn State College of Medicine and external investigators. We currently house the following wild type lines: AB, Conner, Ekkwill, Liles, Tübingen (Tu) and WIK. We also house a selection of mutant and transgenic lines.

Contact Us

For details on the Zebrafish Functional Genomics Core, email Khai Chung Ang, PhD, Scientific Director, at kca2@psu.edu or call 717-531-4704.

Pulmonary Immunology & Physiology Core

The Penn State College of Medicine Pulmonary Immunology & Physiology Core aids investigators in the development and testing of small-animal models to be used for pre-clinical pulmonary research.

Facility Details

The Pulmonary Immunology & Physiology Core is a BSL-2 facility that allows the assessment of pulmonary function related to many clinical situations including airway hyper-responsiveness, ventilation-induced lung injury, postnatal lung development, airway remodeling, chronic inflammation, allergy, environmental exposures and infection.

In addition, services offered include oxygen profiling, infection and treatment; the ability to deliver, measure and test the pre-clinical potential of therapeutic interventions via aerosol; and the housing of small animals used in these studies.

Instrumentation

  • SCIREQ flexiVent FX
  • SCIREQ inExpose
  • Data Sciences International FinePointe Whole Body Plethysmograph
  • STARR Life Sciences MouseOx Plus
  • BioSpherix OxyCycler A84X05
  • Nuaire Biological Safety Cabinet
  • flexiWare Software
  • Data Sciences International FinePointe Software

Contact Us

For details on the Pulmonary Immunology & Physiology Core, email Todd Umstead, Research Project Manager, at tmu1@psu.edu or call 717-531-0003, ext. 284927.

Transgenics & Rederivation

The Transgenic Core Facility at Penn State College of Medicine is a centralized facility for the production of transgenic and knockout mice and the rederivation of mice.

Contact Us

For details on Transgenics & Rederivation, email Ronald P. Wilson, DVM, Director, at rwilson2@pennstatehealth.psu.edu or call 717-531-8460.

Other Services

Bioluminescent Imaging

Penn State College of Medicine has a centralized facility for Bioluminescent Imaging, including capabilities for fluorescent as well as luminescent imaging of intact animals.

The Core has a IVIS Lumina Series III Imaging System with an added Fluorescent Protein module.

For details on Bioluminescent Imaging, contact Sang Lee, PhD, Director, at slee1@pennstatehealth.psu.edu or 717-531-0003, ext. 285546.

Animal Pathology

Penn State College of Medicine's Department of Comparative Medicine offers a Comparative Pathology Service to provide researchers access to experts in veterinary and comparative pathology.

Services available include gross necropsy, histopathology (including semi-quantitative or quantitative analysis), immunohistochemistry, phenotyping, development/implementation of analysis techniques, manuscript review, and clinical pathology.

For details on the comparative pathology service, email Nannette Kirst in the Department of Comparative Medicine at nkirst@pennstatehealth.psu.edu.

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